RBRC00305 Cancer Research C57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB C57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB C (3-6 months) Internal/Organ Research mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Tnf genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse Rela genomic DNA 愛知がんセンター・土井貴弘（1997）。RelAノックアウトマウスとTNFノックアウトマウスとの交配により作出。 C（3〜6か月） covid19_progression 土井　貴裕 Developmental Biology Research C57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB, TNF kappa B C57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB, TNF kappa B Neurobiology Research Produced by corssing relA deficient mice and TNF deficient mice strains. Neomycin cassettes were inserted into the nucleotides 3704-5364 of TNF gene and the downstream of the translation initiation codon of relA gene, respectively. RelA deficient mice were backcrossed to C57BL/6 for 10 generations, and TNF deficient mice were backcrossed to C57BL/6 for 5 generations. Then, the two mutant strains were crossed to create the TNFkB mice. Developed by Takahiro Doi at Aichi Cancer Research Center in 1997. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Endocrine Deficiency Research In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA., 96, 2994-2999 (1999). The for-profit institutions should obtain written confirmation from Ludwig Institute for Cancer Research. Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100<br>Any patent rights and other intellectual properties are retained by the depositor and Ludwig Institute for Cancer Research. Users should contact the depositor and Ludwig Institute for Cancer Research in the case of application for any patents with the results from these mice. W9.5/D3 for TNF alpha, CCE [129/Sv] for relA Tnf遺伝子およびRelA遺伝子をノックアウトしたマウス。RelA遺伝子：リンパ球の発生に関わる因子として発見されたNF-kBファミリーの一つ。全身でくまなく発現している。RelAのみホモノックアウトマウスは胎生期13-14日頃に死亡するが、Tnf/RelAダブルミュータントマウスは生存可能。 Takahiro DOI RIKEN covid19_onset Cardiovascular Research Apoptosis Research Immunology and Inflammation Research Hematological Research 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc Natl Acad Sci USA., 96, 2994-2999 (1999). <br>(1)　会社等が本件リソースの譲渡を希望する場合は、ラドウィックがん研究所にその旨問い合わせる。Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100<br>(2)　本件リソースの特許権ならびに知的所有権は寄託者とラドウィックがん研究所が保持しており、利用者が本件リソースから得られた結果により特許等を申請する場合は、事前に寄託者とラドウィックがん研究所に連絡し、了解を得る。 Diabetes and Obesity Research TNF and RelA double knockout mice. TNF, tumor necrosis factor is a multifunction proinflammatory cytokine. RelA, v-rel reticuloendotheliosis viral oncogene homolog A (Avian) is one of NF-kB family relating to the development of lymphocytes, suggested to be highly sensitive to TNF cytotoxicity. Mice lacking only relA gene die between days 14 and 15 of embryogenesis due to liver cell apoptosis by endogenous TNF cytotoxicity. Meanwhile, TNF/relA double-deficient mice are viable and have normal liver. This strain is useful for studies of functions of RelA in vivo. TNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type TNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type <a href='https://brc.riken.jp/mus/pcr00305'>Genotyping protocol -PCR-</a> true B6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc B6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc 理化学研究所 <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/may_2009_mm" target="_blank">Mouse of the Month May 2009</A>